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NIH ProtIG Seminar by Joshua Coon: How Mass Spectrometry can Drive Biological Discovery – from Structure to Systems

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Air date: Thursday, February 29, 2024, 1:00:00 PM
Time displayed is Eastern Time, Washington DC Local
Views: Total views: 65 (22 Live, 43 On-demand)
Category: Special
Runtime: 01:03:19
Description: In this presentation I will describe the use of a mass spectrometer to prepare samples for cryo-electron microscopy (cryoEM). Briefly the protein-protein complexes are ionized, vaporized, and pass through a modified Orbitrap MS system where they either have their mass measured or are deposited onto a cryogenically cool TEM grid placed at the rear of system. After being deposited, the landed complexes are coated with a thin film of amorphous ice and then removed from the system and placed into a cryoEM for direct imaging. This method has the potential to resolve many outstanding problems with traditional cryoEM grid preparation (i.e., plunge freezing) including air-water interface issues, preferential orientation, and the ability to precisely control ice thickness. Next, I will provide several examples of high throughput proteome, lipidome, and metabolome measurements and how that can help identify protein function and improve human health. Finally, I will present new MS technology for deep human proteome sequencing for detection of RNA editing and protein phosphorylation.

For more information go to https://proteome.nih.gov/
Author: Joshua Coon
 
CIT Live ID: 54400
Permanent link: https://videocast.nih.gov/watch=54400

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